A 3851bp enhancer of shot (from the R38F11 regulatory fragment) drives expression of Cas9 tagged with nuclear localization signals at both the N-terminal and C-terminal ends of the open reading frame.
abnormal stress response | larval stage, with drprU6:3.gRNA
dendrite | larval stage, with drprU6:3.gRNA
larval dorsal multidendritic neuron ddaC, with drprU6:3.gRNA
larval dorsal multidendritic neuron ddaE, with drprU6:3.gRNA