UASt regulates expression of Hsap\NRXN1 bearing the I1135V mutation (found in Autism spectrum disorder patients) with a C-terminal Tag:FLAG tag.
The expression of Hsap\NRXN1I1135V.UAS.Tag:FLAG under the control of Scer\GAL4elav.PU, Scer\GAL4tim.PE or Scer\GAL4P2.4.Pdf leads to circadian defects: Scer\GAL4elav.PU-driven expression leads to increased evening activity, as well as decreased night-time sleep with more sleep bouts, and fewer but longer night-time sleep bouts; Scer\GAL4tim.PE-driven expression induces increased evening activity and increased period, as well as increased day-time sleep, with more sleep bouts, and fewer but longer night-time sleep bouts; Scer\GAL4P2.4.Pdf-driven expression induces a decreased morning index and decreased rhythmicity, as well as more but shorter total and day-time sleep bouts.