An Act88F promoter drives expression of the indirect flight muscle myosin isoform (IFI) of Mhc, mutated to carry a S531P amino acid substitution; this mutation is equivalent to a S532P change in the orthologous human MYH7 gene, a pathogenic variant associated with dilated cardiomyopathy. The coding sequence is composed of cDNA sequence encoding the Mhc IFI motor domain followed by a genomic fragment encompassing Mhc exons 12-19 (and associated introns and polyadenylation sites). The protein is tagged at the N-terminal end with Tag:polyHis and with the Tag:CS(TEVp) cleavage site.
T16776865C
S531P | Mhc-PA; S531P | Mhc-PB; S531P | Mhc-PC; S531P | Mhc-PD; S531P | Mhc-PE; S531P | Mhc-PF; S531P | Mhc-PG; S531P | Mhc-PH; S531P | Mhc-PI; S531P | Mhc-PK; S531P | Mhc-PL; S531P | Mhc-PM; S531P | Mhc-PN; S531P | Mhc-PO; S531P | Mhc-PP; S531P | Mhc-PQ; S531P | Mhc-PR; S531P | Mhc-PS; S531P | Mhc-PT; S531P | Mhc-PU; S531P | Mhc-PV
S531P
Mutation in analagous codon in human MYH7 implicated in cardiomyopathy associated with dilated cardiomyopathy 1S; mutation carried on in vitro construct; specific disease association inferred by FlyBase curator based on reported amino acid change; site of nucleotide change determined from primer sequences.