FB2024_03 , released June 25, 2024
Allele: Amel\ApaminUAS.t.Tag:MYC
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General Information
Symbol
Amel\ApaminUAS.t.Tag:MYC
Species
A. mellifera
Name
FlyBase ID
FBal0369625
Feature type
allele
Associated gene
Amel\Apamin
Associated Insertion(s)
Carried in Construct
P{UAS-t-Apamin.Myc}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Wu et al., 2008)
Progenitor genotype
Carried in construct
P{UAS-t-Apamin.Myc}
Cytology
Description

UAS regulatory sequences drive expression of a fusion protein composed of a membrane-tethered form of the Amel\Apamin ion channel toxin (this toxin targets SK-type Ca[2+]-activated K[+] ion channels). The design of the fusion protein (PMID:15294139) is based on the structure of the lynx1-like gene family, and consists of a Tag:SS(lynx1) secretory signal sequence, the Amel\Apamin toxin peptide, a Gly-Asn repeat hydrophilic linker and a Tag:GPI(lynx1) anchor (this anchor sequence should result in the protein being tethered to the plasma membrane). In addition, the protein is tagged with Tag:MYC. The fusion protein is designed to act as a "tethered-toxin" to allow cell-autonomous modulation of ion channel (and hence neuronal cell) activity, but flies are viable when the protein is expressed pan-neuronally, suggesting that the Amel\Apamin toxin may be non-functional against fly SK-type Ca[2+]-activated K[+] channels (at least in its tethered form).

(Wu et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Amel\Apamin
Tagged with
Tag:GPI(lynx1)
Tag:MYC
Tag:SS(lynx1)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Amel\ApaminUAS.t.Tag:MYC
      Name Synonyms
      Secondary FlyBase IDs
        References (1)