UAS regulatory sequences drive expression of two gRNAs (TTCATCGCACATTGGTTCTC and GCAAGAAGGGACCTGCGCCA) that each target exon 3 of EcR, an exon shared between all EcR isoforms and which encodes the DNA-binding domain. The sgRNAs are expressed as a single polycistronic tRNA-gRNA cassette.