Mutation within the wrm1 open reading frame (ORF), the 5' ORF encoded by the bicistronic wrm locus. The mutation results in a premature stop codon in wrm1. Complementation analysis shows that the function of the wrm2 ORF (the 3' ORF encoded by the bicistronic wrm locus) is unaffected in this mutant.
TAAACTTTGAAGTT
wrm1A homozygous embryos exhibit gas filling failure of tracheal tubes, an elongated tracheal network, diffusion of dextran dyes into the tracheal lumen and disrupted septate junction morphology (irregularly distributed and reduced ladder-like abundance of electron dense material).
wrm1A/wrm1E embryos exhibit diffusion of dextran dyes into the central nervous system.
Avic\GFP[Atpα-ZCL1792] and Avic\GFP[Nrx-IV-CA06597] exhibit fast recovery rates in FRAP experiments in lateral epidermal cells of wrm1A homozygous embryos.