FB2024_03 , released June 25, 2024
Allele: Scer\GAL4ETHR-A.MI00949-TG4.2
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General Information
Symbol
Scer\GAL4ETHR-A.MI00949-TG4.2
Species
S. cerevisiae
Name
FlyBase ID
FBal0358465
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Mi{Trojan-GAL4.2.A}ETHRA.MI00949-TG4.2
Carried in Construct
Also Known As
ETHRA-Gal4, ETHRAMI00949-Gal4
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
phiC31 integrase
(Diao et al., 2016)
Progenitor genotype
Mi{MIC}ETHRMI00949
(Diao et al., 2016)
Associated Insertion(s)
Mi{Trojan-GAL4.2.A}ETHRA.MI00949-TG4.2
Cytology
Description

The RMCE cassette of the Mi{MIC}ETHRMI00949 insertion has been replaced with a construct that duplicates ETHR gene sequence 3' to the insertion site along with fusion of T2A-GAL4 coding sequences to exon 4a of the ETHR gene just prior to the stop codon for ETHR-A. This results in production of full-length ETHR-A and co-expression of GAL4.

(Diao et al., 2016)
Allele components
Component
Use(s)
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      P-stage
      neuron | subset of pupal central nervous system
      (Diao et al., 2017)
      Additional Information
      Statement
      Reference

      ScerGAL4ETHR-A.MI00949-TG4.2 drives expression in a subset of CNS neurons responsive to ecdysis-triggering hormone.

      (Diao et al., 2017)
       
      Marker for
      Reflects expression of
      ETHR
      (Diao et al., 2017)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4ETHR-A.MI00949-TG4.2
      Name Synonyms
      Secondary FlyBase IDs
        References (3)