A sequence cassette has been introduced into the attP site present in TI{TI}NPFRattP, restoring the deleted NPFR sequence and tagging isoforms B and D with 2xTag:MYC. The tag is followed by a 2A-GAL4 cassette, resulting in the GAL4 driver being expressed as a separate protein under the control of the endogenous NPFR regulatory sequences (only the expression of isoforms B and D is trapped by GAL4). A single loxP site is present downstream of the driver sequence (markers present in the progenitor insertion and donor plasmid have been removed using P1cre).