A sequence cassette has been introduced into the attP site present in TI{TI}HdcattP, restoring the deleted Hdc sequence and tagging it in the 7th common exon (before ccggaaggtatgaagcccag) with 2xTag:MYC. The tag is followed by a 2A-GAL4 cassette, resulting in the GAL4 driver being expressed as a separate protein under the control of the endogenous Hdc regulatory sequences. A single loxP site is present downstream of the driver sequence (markers present in the progenitor insertion and donor plasmid have been removed using P1cre).