A sequence cassette has been introduced into the attP site present in TI{TI}AstCattP, restoring the deleted AstC sequence and tagging it with 2xTag:MYC. The tag is followed by a 2A-GAL4 cassette, resulting in the GAL4 driver being expressed as a separate protein under the control of the endogenous AstC regulatory sequences. A single loxP site is present downstream of the driver sequence (markers present in the progenitor insertion and donor plasmid have been removed using P1cre).
ScerGAL4AstC-2A-GAL4 drives expression in a large number of enteroendocrine cells of the middle (R3) and posterior (R4) regions and in a smaller number in the anterior R2 region of the adult midgut.
Scer\GAL4AstC-2A-GAL4, Scer\GAL80R57C10, TrpA1UAS.(B).cKa has increased rate of feeding behavior phenotype, suppressible by AstCUAS.KO.pCFD6/Spyo\Cas9UAS.P2, Scer\GAL4AstC-2A-GAL4, Scer\GAL80R57C10
AstCUAS.KO.pCFD6/Spyo\Cas9UAS.P2, Scer\GAL80R57C10, Scer\GAL4AstC-2A-GAL4 is a suppressor of increased rate of feeding behavior phenotype of Scer\GAL4AstC-2A-GAL4, Scer\GAL80R57C10, TrpA1UAS.(B).cKa