A sequence cassette has been introduced into the attP site present in TI{TI}VGlutattP, restoring the deleted VGlut sequence, followed by a 2A-p65(AD)::Zip+ cassette, resulting in the p65(AD)::Zip+ hemidriver being expressed as a separate protein under the control of the endogenous VGlut regulatory sequences. A single loxP site is present downstream of the hemidriver sequence (markers present in the progenitor insertion and donor plasmid have been removed using P1cre).