The coding sequence of the first coding exon of Dop1R2 has been replaced with a sequence cassette that encodes GAL4, allowing expression of the driver under the control of endogenous Dop1R2 regulatory sequences. A loxP cassette containing a GMR::w marker has also been inserted, downstream of the GAL4 sequence.
Dop1R2KOGal4 homozygous adults exhibit a significant increase in sleep during day time (with significantly increased bout number and duration) and night time (with significantly increased bout number but not duration) but a significant reduction in sleep recovery rate after mechanical deprivation when compared to controls.