FlyBase Allele Report: Dmel\tra[hs.PM]

General Information

Symbol

Dmel\tra^hs.PM

Species

D. melanogaster

Name

heat shock construct of McKeown

FlyBase ID

FBal0355628

Feature type

allele

Associated gene

Dmel\tra

Associated Insertion(s)

Carried in Construct

P{hs-tra.F.cDNA}

Also Known As

hs-tra

Key Links

Transgenic product class

cDNA

(McKeown et al., 1988)

wild_type

(McKeown et al., 1988)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(McKeown et al., 1988)

wild_type

(McKeown et al., 1988)

Mutagen

in vitro construct

(McKeown et al., 1988)

Progenitor genotype

Carried in construct

P{hs-tra.F.cDNA}

Cytology

Description

An Hsp70 promoter drives expression of a tra female cDNA.

(McKeown et al., 1988)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

tra

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Finley et al., 1997)

abnormal sex-determination

(Andrews and Oliver, 2002, Hinson and Nagoshi, 1999)

abnormal sex-determination | dominant

(Taylor, 1992)

abnormal sex-determination | male

(Feng et al., 1991)

Phenotype Manifest In

male genitalia

(Feng et al., 1991)

male reproductive system

(Hinson et al., 1999)

ovary

(Andrews and Oliver, 2002)

spermatocyte fusome

(Hinson et al., 1999)

spermatocyte ring canal

(Hinson et al., 1999)

Detailed Description

Statement

Reference

1X flies expressing tra^hs.PM show male to female somatic transformation. Tumorous ovaries are seen in these animals.

(Andrews and Oliver, 2002)

At the tips of germaria of XYtra^hs.PM ovaries are found apically located spherical spectrosomes which sometimes exhibit the typical tail-like structure. In more posterior regions, cells of ovaries of adult XYtra^hs.PM pseudofemales mostly have branched fusome structures that connect up to 26 cells. In some cases, spectrosomes are also detected.

(Janzer and Steinmann-Zwicky, 2001)

XY flies expressing tra^hs.PM develop as pseudofemales.

(Hinson and Nagoshi, 1999)

In XY pseudofemales, produced by the heat shock induction of tra^hs.PM, gametogenesis is disrupted during the cystocyte divisions, but oogenic differentiation is not induced. In XY pseudofemales, all egg chambers contain germ cell clusters with spectrosomes or fusomes. The most mature germ cells are interconnected by multibranched fusomes (polyfusomes) containing f-actin, phenotype suggestive of spermatogenic differentiation. More aberrant germ cells are also found in small (2-3 cell) clusters connected by short, poorly branched fusomes. The retention of f-actin in this subset is variable. All this indicates an early defect in the spectrosome to fusome transition that occurs at the first cystocyte division. In XY pseudofemales, aberrations are also found in the composition of the ring canals.

(Hinson et al., 1999)

tra[-] females carrying tra^hs.PM have a reduced number of boutons on the circular uterine muscles.

(Finley et al., 1997)

60% of tra^hs.PM XY females have large numbers of male germ cells.

(Oliver et al., 1994)

X/Y; tra^hs.PM first instar larvae contain gonads of male size, but by the end of the third larval instar their size is comparable to that of the third larval instar. Morphology of the germ cells is male-like. X/X; tra¹/tra¹; tra^hs.PM first instar larvae have masculinized gonads, though this phenotype is variable.

(Steinmann-Zwicky, 1994)

At 25[o]C the sex specific muscle of female flies bearing tra^hs.PM allele remains male-like: failure to feminize the male specific muscle may be due to insufficient levels or inappropriate expression to make functional germ cells.

(Taylor, 1992)

XY flies expressing tra^hs.PM have female somatic reproductive organs.

(Feng et al., 1991)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

ovo[-],tra^hs.PM XY ovaries have abundant germ cells.

(Oliver et al., 1994)

Sxl^M1,f3/Sxl^f7,M1; tra^hs.PM individuals develop as females, with normal ovaries with oogenic cells, though resulting eggs are not laid. None of the ovaries contain spermatogenic cells.

(Steinmann-Zwicky, 1994)

vir^ts/vir^ts tra^hs.PM/+ XX flies are phenotypically female at 29[o]C, suggesting that vir acts upstream of tra. These animals are sterile when tested at 29[o]C and 25[o]C.

(Hilfiker and Nothiger, 1991)

Xenogenetic Interactions

Statement

Reference

X[[mel]]/X[[sim]]; tra^hs.PM hybrids are recovered in crosses of D. melanogaster females to D. simulans males. X[[mel]]/Y[[sim]]; tra^hs.PM hybrids are not recovered in these crosses. X[[mel]]/Y[[sim]]; tra^hs.PM phenotypically female hybrids are recovered if the D. simulans male parent is carrying Dsim\Lhr^unspecified.

(Orr, 1999)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

tra^hs.PM

(Andrews and Oliver, 2002)

Name Synonyms

heat shock construct of McKeown

Secondary FlyBase IDs

References (18)

Report Sections

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