T2A peptide (supports ribosomal skipping) and GAL4(DBD)::Zip- (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain) coding sequence is inserted immediately preceding the HGTX stop codon.
The T2A peptide (supports ribosomal skipping) and GAL4(DBD)::Zip- (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain) coding sequence is inserted (by knock-in) immediately preceding the HGTX stop codon.