FB2024_03 , released June 25, 2024
Allele: Hsap\RELAAD.VT022025
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General Information
Symbol
Hsap\RELAAD.VT022025
Species
H. sapiens
Name
FlyBase ID
FBal0344099
Feature type
allele
Associated gene
Hsap\RELA
Associated Insertion(s)
Carried in Construct
P{VT022025-p65.AD}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(O'Sullivan et al., 2018)
Progenitor genotype
Carried in construct
P{VT022025-p65.AD}
Cytology
Description

The VT022025 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).

(O'Sullivan et al., 2018)
Allele components
Component
Use(s)
Regulatory region(s)
VT022025
Encoded product / tool
p65(AD)::Zip+
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      wing axillary muscle hg2 motor neuron

      Comment: when combined with P{VT019911-GAL4.DBD} (combination referred to as 'hg2-SG2')

      (O'Sullivan et al., 2018)
      dorsal tp motor neuron

      Comment: when combined with P{VT029310-GAL4.DBD} (combination referred to as 'tp1-SG')

      (Dickerson et al., 2019, O'Sullivan et al., 2018)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Silencing (using Ctet\TeTxLCTNT.UAS) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.VT029310 and Hsap\RELAAD.VT022025 in adult males results in a significant reduction in the number of males that sing courtship song to females, with significant alterations in some parameters of pulse song, but not sine song, compared to controls; there is no significant effect on wing extension toward females or copulation success; take off and forced flight ability are significantly impaired, compared to controls.

      Silencing (using Ctet\TeTxLCTNT.UAS) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.VT019911 and Hsap\RELAAD.VT022025 in adult males significantly alters some parameters of pulse song and sine song, compared to controls; a similar number of males sing and wing extension toward females is unaffected, but copulation success is significantly reduced compared to controls; take off, but not forced flight, ability is significantly impaired compared to controls.

      (O'Sullivan et al., 2018)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Hsap\RELAAD.VT022025
      Name Synonyms
      Secondary FlyBase IDs
        References (2)