FB2024_03 , released June 25, 2024
Allele: Dmel\chkCRSPR
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General Information
Symbol
Dmel\chkCRSPR
Species
D. melanogaster
Name
FlyBase ID
FBal0337756
Feature type
allele
Associated gene
Dmel\chk
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Delgado et al., 2018)
Progenitor genotype
Cytology
Description

A small deletion early in the coding sequence that causes a frame-shift and the premature termination of the chk protein without a destabilization of the mRNA - this results in a specific decrease of chk (without an effect in the second ORF (CG45092) of this dicistronic mRNA.

(Delgado et al., 2018)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
2R:6,704,373..6,704,389 [-]
Comment:

A 17 bp deletion shortly after the chk ATG causes a frameshift and early translation termination after the addition of 10 novel amino acids.

(Delgado et al., 2018)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      chkCRSPR as well as chkCRSPR/Df(2R)BSC326 mutants do not show any obvious defects in the gross adult brain morphology or any deficit in larval locomotion (assessed by speed of crawling and frequency of contraction) but display significant decrease in adult climbing ability. chkCRSPR homozygotes exhibit synaptic transmission defects (increased amplitude of evoked postsynaptic currents at 1 or 2 mM external Ca[2+], no difference in amplitude is observed at low calcium levels) at larval neuromuscular junctions, have reduced adult survival rate and lower resistance to starvation (also observed with chkMB04207/chkCRSPR transheterozygotes) as compared to controls.

      (Delgado et al., 2018)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      ChkCRISPR
      (González-Gutiérrez et al., 2020)
      chkCRSPR
      (Delgado et al., 2018)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)