Insertion of a genomic Cirl fragment at the attP site in CirlKO.attP.w-; the fragment contains a T>A mutation in the GPCR autoproteolysis inducing (GAIN) domain, to abolish autoproteolytic activity; also contains a w+* marker.
The responses of CirlT-A mutant lch5 neurons in larvae to mechanical stimulation are reduced, compared to controls.