CRISPR construct designed to result in an indel (small insertion or deletion mutation) in a target gene when combined with a source of Cas9. Constitutively expresses an sgRNA that targets the coding sequence of whd.
Expression of whdTKO.GS00854 under the control of Scer\GAL4dome-PG14 (in combination with Spyo\Cas9UAS.cUa and tub-Gal80[ts]) leads to a decline in the cortical zone of lymph gland primary lobe of larvae.