CRISPR construct designed to result in an indel (small insertion or deletion mutation) in a target gene when combined with a source of Cas9. Constitutively expresses an sgRNA that targets the coding sequence of Graf.
CRISPR/CAS9 mutagenesis of Graf in the female germline (GrafTKO.GS00762, Spyo\Cas9nos.PR) leads to cellularization embryos displaying contractile ring hyper-constriction.