The endogenous TBPH coding region is replaced with fly codon-optimized Hsap\TARDBP cDNA with an N-terminal Tag:FLAG tag and a Disc\RFPDsRed.3xP3.cUa marker flanked by loxP sites at the C-terminal. This results in the Hsap\TARDBP sequence being expressed under the control of the endogenous TBPH regulatory regions.
The phenotype caused by this allele can be ameliorated by expression of P{UAS-gypsy.dsRNA}, which was reported by the authors as UAS-mdg4-IR.