FlyBase Allele Report: Scer\GAL4[DBD.R65D05]

General Information

Symbol

Scer\GAL4^DBD.R65D05

Species

S. cerevisiae

Name

FlyBase ID

FBal0332228

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{R65D05-GAL4.DBD}

Also Known As

R65D05-DBD

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Rubin Laboratory at Janelia Farm, 2017)

Progenitor genotype

Carried in construct

P{R65D05-GAL4.DBD}

Cytology

Description

The GMR65D05 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).

(Rubin Laboratory at Janelia Farm, 2017)

Allele components

Component

Use(s)

Regulatory region(s)

GMR65D05

Encoded product / tool

GAL4(DBD)::Zip-

split driver - DNA-binding fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

neuron of larval descending neuron to VNC

Comment: when combined with P{R56H09-p65.AD} (combination referred to as 'SS01650')

(Tastekin et al., 2018)

adult stage

descending neuron of the gnathal ganglion DNg27

Comment: when combined with P{R13D04-p65.AD} (combination referred to as 'SS01557')

(Namiki et al., 2018)

proximal medullary amacrine neuron Pm3

Comment: when combined with P{R70B07-p65.AD} (combination referred to as 'SS00328')

(Davis et al., 2020)

adult LP neuron | subset

Comment: when combined with P{R11B03-p65.AD}

(Sekiguchi et al., 2020)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal chemosensory behavior | larval stage, with Ctet\tetX^TNT-LC.UAS, Hsap\RELA^AD.R56H09

(Tastekin et al., 2018)

abnormal locomotor behavior | larval stage, with Ctet\tetX^TNT-LC.UAS, Hsap\RELA^AD.R56H09

(Tastekin et al., 2018)

Phenotype Manifest In

Detailed Description

Statement

Reference

Inhibition (using Ctet\TeTxLC^TNT.UAS) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R65D05 and Hsap\RELA^AD.R56H09 in third instar larvae significantly affects chemotactic performance, compared to controls.

(Tastekin et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions