FlyBase Allele Report: Scer\GAL4[DBD.R28A10]

General Information

Symbol

Scer\GAL4^DBD.R28A10

Species

S. cerevisiae

Name

FlyBase ID

FBal0331612

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{R28A10-GAL4.DBD}

Also Known As

R28A10

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Rubin Laboratory at Janelia Farm, 2017)

Progenitor genotype

Carried in construct

P{R28A10-GAL4.DBD}

Cytology

Description

The GMR28A10 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).

(Rubin Laboratory at Janelia Farm, 2017)

Allele components

Component

Use(s)

Regulatory region(s)

GMR28A10

Encoded product / tool

GAL4(DBD)::Zip-

split driver - DNA-binding fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval mushroom body output neuron h1

Comment: when combined with P{R20A02-p65.AD} (combination referred to as 'SS00928')

when combined with P{R20A02-p65.AD} (combination referred to as 'SS01725')

(Truman et al., 2023, Saumweber et al., 2018)

larval mushroom body output neuron h2

Comment: when combined with P{R20A02-p65.AD} (combination referred to as 'SS00928')

when combined with P{R20A02-p65.AD} (combination referred to as 'SS01725')

(Truman et al., 2023, Saumweber et al., 2018)

adult stage

adult lateral horn PV5b3 neuron

Comment: when combined with P{R11E08-p65.AD} (combination referred to as 'LH849')

(Dolan et al., 2019)

adult lateral horn PV5c1 neuron

Comment: when combined with P{R16C09-p65.AD} (combination referred to as 'LH173')

when combined with P{R19E06-p65.AD} (combination referred to as 'LH843')

when combined with P{R89A01-p65.AD} (combination referred to as 'LH178')

(Dolan et al., 2019)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Inhibition (using Hsap\KCNJ2^5XUAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R28A10 and Hsap\RELA^AD.R20A02 in third instar larvae does not significantly affect short-term associative appetitive olfactory memory following training with fructose, innate odor (n-amylacetate) preference or innate fructose preference, compared to controls.

(Saumweber et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions