FlyBase Allele Report: Scer\GAL4[DBD.R27A11]

General Information

Symbol

Scer\GAL4^DBD.R27A11

Species

S. cerevisiae

Name

FlyBase ID

FBal0331598

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{R27A11-GAL4.DBD}

Also Known As

R27A11

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Rubin Laboratory at Janelia Farm, 2017)

Progenitor genotype

Carried in construct

P{R27A11-GAL4.DBD}

Cytology

Description

The GMR27A11 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).

(Rubin Laboratory at Janelia Farm, 2017)

Allele components

Component

Use(s)

Regulatory region(s)

GMR27A11

Encoded product / tool

GAL4(DBD)::Zip-

split driver - DNA-binding fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval mushroom body dopaminergic neuron k1

Comment: when combined with P{R48F09-p65.AD} (combination referred to as 'SS01757')

(Truman et al., 2023, Saumweber et al., 2018)

adult stage

adult neuron | subset of adult brain

Comment: when combined with P{R76E09-p65.AD} (combination referred to as 'SS43334')

(Sterne et al., 2021)

adult pringle subesophageal neuron

Comment: when combined with P{R76E09-p65.AD} (combination referred to as 'SS43334')

when combined with P{VT036871-p65.AD} (combination referred to as 'SS43335')

(Sterne et al., 2021)

adult brontosaraus subesophageal neuron

Comment: when combined with P{VT008686-p65.AD} (combination referred to as 'SS40950')

(Sterne et al., 2021)

adult neuron | subset of adult prothoracic neuromere | subset

Comment: when combined with P{VT036871-p65.AD} (combination referred to as 'SS43335')

(Sterne et al., 2021)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal chemosensory behavior | larval stage, with Hsap\KCNJ2^5XUAS.EGFP, Hsap\RELA^AD.R48F09

(Saumweber et al., 2018)

Phenotype Manifest In

Detailed Description

Statement

Reference

Inhibition (using Hsap\KCNJ2^5XUAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R27A11 and Hsap\RELA^AD.R48F09 in third instar larvae does not significantly affect short-term associative appetitive olfactory memory following training with fructose, but does reduce innate odor (n-amylacetate) preference and innate fructose preference, compared to controls.

Optogenetic activation (using Crei\ChR2^XXL.UAS or Crei\ChR2^{XXL.UAS.tdTomato} with 470 nm (blue) light or using Cnoc\ChR1^{Cs.IVS.20xUAS.Venus} with 630 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R27A11 and Hsap\RELA^AD.R48F09 in third instar larvae, in combination with a paired odor, does not result in a significant difference in short-term associative olfactory memory, compared to controls.

(Saumweber et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions