The GMR20G04 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).
Comment: when combined with PBac{R73H08-p65.AD} (combination referred to as 'MB091C')
Inhibition (using shits1.IVS.p10.20xUAS at 31-33[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R20G04 and Hsap\RELAAD.R73H08 in adults only during training and testing significantly reduces 24 h appetitive (to the extent that this is aversive), but not 30 min aversive, associative olfactory memory towards ethanol, compared to controls.