The GMR14H05 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).
Thermogenetic activation (using TrpA1UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R14H05 and Hsap\RELAAD.R19B03 in adult males or females does not significantly affect the amount of sleep, compared to controls; activation does not significantly affect locomotor activity of awake adult males, compared to controls.