The GMR14A06 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).
Comment: when combined with P{R10B11-p65.AD} (combination referred to as '10B11-AD ∩ 14A06-DBD')
Comment: when combined with P{R10B11-p65.AD} (combination referred to as '10B11-AD ∩ 14A06-DBD')
Ablation (using hidUAS.cZa) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R14A06 and Hsap\RELAAD.R10B11 frequently results in the giant fiber interneuron (GFI) being lost from one hemisphere, with the remaining GFI extending a compensatory axon to the contralateral side and forming a terminal axon bend.