The expression of NctdsRNA.shRNA.Scer\UAS.3 under the control of Scer\GAL4Act5C.PU leads to early-pupal lethality, as compared to control; expression under the control of Scer\GAL4elav-C155 leads to pupal lethality in males and semi-lethality in females, but the surviving adults show a severely decreased lifespan, severe locomotor defects in negative geotaxis assays, and a rough eye phenotype; expression under the control of Scer\GAL4bbg-C96 leads to wing defects, ranging from severe wing vein and blistering phenotypes to wing margin loss, as compared to controls.