C to T nucleotide substitution introduces a premature stop codon, resulting in a truncated peptide.
Point mutation introduces a stop codon in exon 3 resulting in a truncated Rx protein of 230 aa, missing the homeodomain.
C20922639T
Inferred location of a nonsense mutation that is reported as a C to T change resulting in a truncated Rx protein of 233 amino acids. The mapped change is in codon 235. A C to T change in codon 234 would not lead to a nonsense mutation.
Rx10155/Rx10155 mutant embryos do not show a difference in the number of mushroom body neuroblasts at stage 17, but the neuroblasts are significantly reduced in size; these mutants also show a significant decrease in the number of mushroom body neuroblast daughter cells in the mushroom body at stage 17, and a marked decrease in mitotic index of mushroom body neuroblasts in late embryonic stages, as compared to wild type.
Rx10155/Rx10155 mushroom body neuroblast (NB) clones in third instar larvae and mid-pupal stages show a significant reduction in overall cell number, a significant decrease in the number of mushroom body GMCs, a decrease in frequency of mitotic mushroom body neuroblasts, and a decrease in mushroom body NB size, as compared to wild type clones. At third instar larval stages and midpupal stages, Rx10155/Rx10155 mushroom body NB clones show a significant decrease in mushroom body NB number as compared to wild type, and a complete loss of mushroom body NBs at 72 h APF, in contrast to controls.
