Pif1AZ3-5009 homozygotes exhibit spermatogenesis defects: unlike controls, no actin cones progresses away from the nuclei; actin clones close to the spermatid nuclei exhibit shorter and thinner actin filaments than the controls; some actin cones appear to move slightly away from the nuclei and have wider fronts and narrower rears; no cystic bulges/waste bags are observed; there are numerous axonemes and mitochondria within a single membrane, and the axonemes are not tightly juxtaposed to the mitochondria, indicating failure in spermatid individualization; individualizing cysts appear thin and wispy,
Mutant males are defective in spermatid individualization.
Pif1AZ3-5009 is rescued by Pif1AHsp83.RI.GFP