Flies expressing NKCC^{Scer\UAS.T:Zzzz\FLAG,T:Ivir\HA1} under the control of Scer\GAL4^tim.PE or Scer\GAL4^qsm-NP6258 exhibit long-period locomotor rhythms in constant dim white light, in contrast to control flies which largely show arrhythmic behavior in these conditions. Flies expressing NKCC^{Scer\UAS.T:Zzzz\FLAG,T:Ivir\HA1} under the control of Scer\GAL4^tim.PE do not exhibit any difference in locomotor rhythms when kept in constant darkness, as compared to controls.

The l-LNv neurons in flies expressing NKCC^{Scer\UAS.T:Zzzz\FLAG,T:Ivir\HA1} under the control of Scer\GAL4^Pdf.PU exhibit depolarization of the nighttime (but not daytime) resting membrane potential, and an increased spontaneous firing rate during the night (but not during the day), as compared to wild type; these neurons also show a heightened response to blue light when tested during the daytime, as compared to wild type.