UASt regulatory sequences drive expression of dco in which the nuclear localization signal sequence has been mutated (from KRQK to NNQN). The protein is tagged at the C-terminal end with Tag:MYC.
The expression of dcoNLS-.UAS.Tag:MYC under the control of Scer\GAL4tim.PE leads to a significant decrease in circadian period, but has no obvious effect in circadian rhythmicity, as compared to controls.