UAS regulatory sequences drive expression of the Zasp52-PK isoform, tagged at the N-terminal end with a Tag:FLAG-Tag:StrepII-Tag:polyHis tag cassette.
Expression of Zasp52Scer\UAS.PK.FSH under the control of Scer\GAL4Mef2.PU results in indirect flight muscle defects, as compared to controls.
Expression of Zasp52Scer\UAS.PK.FSH under the control of Scer\GAL4Hk-UH3 fully rescues the indirect flight muscles seen in Zasp52MI02988/Df(2R)BSC427 mutants.