FlyBase Allele Report: Mmus\Nos2[Mac.UAS]

General Information

Symbol

Mmus\Nos2^Mac.UAS

Species

M. musculus

Name

FlyBase ID

FBal0321377

Feature type

allele

Associated gene

Mmus\Nos2

Associated Insertion(s)

Carried in Construct

P{UAS-mNos2.Mac}

Also Known As

UAS-macNOS

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Cáceres et al., 2011)

Progenitor genotype

Carried in construct

P{UAS-mNos2.Mac}

Cytology

Description

UAS regulatory sequences drive expression of a constitutively active form of Mmus\Nos2.

(Cáceres et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mmus\Nos2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of epilepsy

CEA

(Giachello et al., 2021)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian rhythm | adult stage, with Scer\GAL4^GMR33H10

(Kozlov et al., 2020)

abnormal circadian rhythm | adult stage, with Scer\GAL4^GMR57C10

(Kozlov et al., 2020)

abnormal circadian rhythm | adult stage, with Scer\GAL4^GMR79D04

(Kozlov et al., 2020)

abnormal circadian rhythm | adult stage, with Scer\GAL4^GMR85B12

(Kozlov et al., 2020)

abnormal circadian rhythm | adult stage, with Scer\GAL4^P2.4.Pdf

(Kozlov et al., 2020)

abnormal circadian rhythm | adult stage, with Scer\GAL4^repo

(Kozlov et al., 2020)

abnormal circadian rhythm | adult stage, with Scer\GAL4^tim.PU

(Kozlov et al., 2020)

abnormal developmental rate, with Scer\GAL4^phtm.PO

(Cáceres et al., 2011)

abnormal neuroanatomy | P-stage, with Scer\GAL4^elav-C155

(Rabinovich et al., 2016)

abnormal neuroanatomy | P-stage, with Scer\GAL4^ey-OK107

(Rabinovich et al., 2016)

decreased body size | larval stage, with Scer\GAL4^phtm.PO

(Cáceres et al., 2011)

lethal - all die before end of larval stage, with Scer\GAL4^phtm.PO

(Cáceres et al., 2011)

some die during larval stage, with Scer\GAL4^phtm.PO

(Cáceres et al., 2011)

Phenotype Manifest In

adult mushroom body gamma-lobe | P-stage, with Scer\GAL4^elav-C155

(Rabinovich et al., 2016)

adult mushroom body gamma-lobe | P-stage, with Scer\GAL4^ey-OK107

(Rabinovich et al., 2016)

adult s-LNv neuron, with Scer\GAL4^P2.4.Pdf

(Kozlov et al., 2020)

axon | P-stage, with Scer\GAL4^elav-C155

(Rabinovich et al., 2016)

axon | P-stage, with Scer\GAL4^ey-OK107

(Rabinovich et al., 2016)

vesicle, with Scer\GAL4^phtm.PO

(Cáceres et al., 2011)

Detailed Description

Statement

Reference

Expression of Mmus\Nos2^Mac.Scer\UAS under the control of Scer\GAL4^ey-OK107 or Scer\GAL4^elav-C155 promotes abnormally early axon pruning in the mushroom body gamma-lobe, as compared to controls.

(Rabinovich et al., 2016)

Larvae in which constitutively active Mmus\Nos2^Mac.Scer\UAS is expressed under the control of Scer\GAL4^phm.PO eat less than their wild-type contemporaries and grow more slowly, failing to pass beyond the second instar larval stage (as determined by mouth-hook teeth numbers). The majority of these larvae die by 5 days after egg laying.

The prothoracic glands in embryos expressing Mmus\Nos2^Mac.Scer\UAS under the control of Scer\GAL4^phm.PO are comparable in size to same-age wild-type larval prothoracic glands, as are the ratios between prothoracic gland and brain lobe diameters.

The small size of larvae expressing Mmus\Nos2^Mac.Scer\UAS under the control of Scer\GAL4^phm.PO appears to be attributed to a decrease in the size of all tissues. While the relative size of the fat bodies in these larvae appear to be the same as in wild-type, lipid vesicles are notably depleted of triglycerides.

(Cáceres et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Mmus\Nos2^Mac.UAS, Scer\GAL4^R6 has abnormal circadian rhythm | adult stage phenotype

(Kozlov et al., 2020)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Mmus\Nos2^Mac.Scer\UAS

Mmus\Nos2^Mac.UAS

Nos^Mac.Scer\UAS

Name Synonyms

mouse macrophage Nos

(Cáceres et al., 2011)

Secondary FlyBase IDs

FBal0320398
FBal0263809

References (4)

Report Sections

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