Amino acid replacement: Q513term.
C17071640T
Q513term | wcy-PD; Q513term | wcy-PE; Q513term | wcy-PA; Q513term | wcy-PC; Q555term | wcy-PF; Q513term | wcy-PG
Q513term
Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
Electroretinograms (ERGs) recorded from wcyC/wcyC mutant eye clones show significantly reduced amplitudes and off transients compared to controls; at day 1, mutant clones show disruption of ommatidial units, with abnormally fused or split rhabdomeres and loss of photoreceptor cells and mutant clones also show show age-dependent (1 day old versus 36 days old) degeneration of internal eye structures (defects are more severe than day 1 and numerous photoreceptors are lost, leaving vacuoles). One day old mutant lamina clones show an increase in autophagosomes, amphisomes, autolysosomes and lysosomes compared to controls.
wcyC is rescued by Scer\GAL4Ubi.PU/wcyUAS.cDa
wcyC is rescued by Scer\GAL4Ubi.PU/wcyUAS.Tag:FLAG
wcyC is rescued by wcyTag:FLAG,Tag:HA
Expression of wcyScer\UAS.cDa or wcyScer\UAS.T:Zzzz\FLAG driven by Scer\GAL4Ubi.PU or wcy+tGR, wcyT:Zzzz\FLAG,T:Ivir\HA1 or wcyT:Avic\GFP-EGFP rescues lethality in wcyC/wcyC mutants. wcy+tGR rescues electroretinogram defects in wcyC/wcyC mutants.