FB2024_03 , released June 25, 2024
Allele: Hsap\MAPTUAS.2N4R.wt
Open Close
General Information
Symbol
Hsap\MAPTUAS.2N4R.wt
Species
H. sapiens
Name
FlyBase ID
FBal0319133
Feature type
allele
Associated gene
Hsap\MAPT
Associated Insertion(s)
Carried in Construct
P{UAS-hTau.2N4R.wt}
Key Links
Transgenic product class
wild_type
(Gorsky et al., 2016)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Gorsky et al., 2016)
Mutagen
in vitro construct
(Gorsky et al., 2016)
Progenitor genotype
Carried in construct
P{UAS-hTau.2N4R.wt}
Cytology
Description

UASt regulatory sequences drive expression of the full-length (441 amino acid residue) wild-type 2N4R isoform of Hsap\MAPT.

(Gorsky et al., 2016)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Hsap\MAPT
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  tauopathy
      CEA
      (Zhu et al., 2022, Gorsky et al., 2016)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Adult-onset expression of Hsap\MAPTScer\UAS.2N4R.wt (two copies) driven by Scer\GAL4elav.Switch.PO (feeding adult flies RU486) leads to a significantly shorter lifespan compared to controls at 29[o]C; driving a single copy of Hsap\MAPTScer\UAS.2N4R.wt at 29[o]C (but not 25[o]C) also significantly reduces lifespan but to a lesser degree. Flies with adult-onset (feeding adult flies RU486) expression of Hsap\MAPTScer\UAS.2N4R.wt driven by Scer\GAL4elav.Switch.PO have significant locomotor (climbing ability) defects at 10 and even more so at 15 days old, and show progressive loss of rhabdomeres (around 15% of ommatidia lack at least one rhabdomere by day 27) compared to controls.

      Flies with adult-onset (feeding adult flies RU486) expression of Hsap\MAPTK280Q.Scer\UAS.2N4R driven by Scer\GAL4elav.Switch.PO show progressive loss of rhabdomeres (around 30% of ommatidia lack at least one rhabdomere by day 27 and degeneration is detectable already at day 9), significantly more degeneration than is seen with expression of Hsap\MAPTScer\UAS.2N4R.wt or Hsap\MAPTK280R.Scer\UAS.2N4R (expression of either of these lines leads to similar amounts of progressive rhabdomere loss).

      Clearance of Hsap\MAPT (2 days of RU486 feeding to induce expression followed by 2 to 5 days without RU486) is significantly slower with expression of Hsap\MAPTK280Q.Scer\UAS.2N4R driven by Scer\GAL4elav.Switch.PO, compared to expression of Hsap\MAPTScer\UAS.2N4R.wt or Hsap\MAPTK280R.Scer\UAS.2N4R.

      Locomotor defects (at 13 days old) are significantly less severe in flies with expression of Hsap\MAPTK280R.Scer\UAS.2N4R compared to expression of Hsap\MAPTK280Q.Scer\UAS.2N4R or Hsap\MAPTScer\UAS.2N4R.wt (driven by Scer\GAL4elav.Switch.PO, with RU486 feeding of adults).

      Lifespan is significantly shorter in flies with expression of Hsap\MAPTScer\UAS.2N4R.wt driven by Scer\GAL4elav.Switch.PO (with RU486 feeding of adults), compared to controls.

      (Gorsky et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Hsap\MAPTScer\UAS.2N4R.wt
      Hsap\MAPTUAS.2N4R.wt
      Name Synonyms
      Secondary FlyBase IDs
        References (2)