FB2024_03 , released June 25, 2024
Allele: Dmel\TetMI05009-GFSTF.0
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General Information
Symbol
Dmel\TetMI05009-GFSTF.0
Species
D. melanogaster
Name
FlyBase ID
FBal0318615
Feature type
allele
Associated gene
Dmel\Tet
Associated Insertion(s)
Mi{PT-GFSTF.0}TetMI05009-GFSTF.0
Carried in Construct
Key Links
Allele class

wild-type allele

Mutagen
Nature of the Allele
Allele class

wild-type allele

(Wang et al., 2018)
Mutagen
phiC31 integrase
(Delatte et al., 2016)
Progenitor genotype
Mi{MIC}TetMI05009
(Delatte et al., 2016)
Associated Insertion(s)
Mi{PT-GFSTF.0}TetMI05009-GFSTF.0
Cytology
Description

The RMCE cassette of the Mi{MIC}TetMI05009 insertion has been replaced with a EGFP-FlAsH-StrepII-TEV-3xFlag multi-tag cassette, resulting in a GFP-tagged version of Tet.

(Delatte et al., 2016)
Allele components
Component
Use(s)
Inserted element
Mi{PT-GFSTF.0}
Tagged with
EGFP
Tag:CALI(TC)
Tag:CS(TEVp)
Tag:FLAG
Tag:StrepII
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter or direct label
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      larval stage
      embryonic/larval brain
      (Delatte et al., 2016)
      embryonic/larval optic lobe
      (Delatte et al., 2016)
      Additional Information
      Statement
      Reference

      GFP-tagged Tet protein is detected throughout the larval brain, with the highest levels in the optic lobe and central brain.

      (Delatte et al., 2016)
       
      Marker for
      Reflects expression of
      Tet
      (Delatte et al., 2016)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      TetMI05009-GFSTF.0
      Name Synonyms
      Secondary FlyBase IDs
        References (3)