FB2024_03 , released June 25, 2024
Allele: Hsap\RELAAD.VT050405
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General Information
Symbol
Hsap\RELAAD.VT050405
Species
H. sapiens
Name
FlyBase ID
FBal0316908
Feature type
allele
Associated gene
Hsap\RELA
Associated Insertion(s)
Carried in Construct
P{VT050405-p65.AD}
Also Known As
VT50405-p65AD, VT050405-p65ADZp
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Feng et al., 2014)
Progenitor genotype
Carried in construct
P{VT050405-p65.AD}
Cytology
Description

The VT050405 enhancer fragment regulates expression of a p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).

(Feng et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
VT050405
Encoded product / tool
p65(AD)::Zip+
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage | female
      adult central nervous system | restricted

      Comment: when combined with P{VT045154-GAL4.DBD}

      (Feng et al., 2014)
      adult post-embryonic ventral posterior Ilp7 (female) neuron

      Comment: when combined with P{VT045154-GAL4.DBD}

      (Feng et al., 2014)
      posterior superior medial protocerebrum | restricted

      Comment: when combined with P{VT045154-GAL4.DBD}

      (Feng et al., 2014)
      adult stage
      adult sex peptide abdominal ganglion neuron | subset

      Comment: when combined with P{VT007068-GAL4.DBD} (combination referred to as 'SAG-GAL4')

      (Jang et al., 2017)
      adult sex peptide abdominal ganglion neuron

      Comment: when combined with P{VT007068-GAL4.DBD} (combination referred to as 'SAG-SS')

      when combined with P{VT007068-GAL4.DBD} (combination referred to as 'SAG1-GAL4')

      when combined with P{VT007068-GAL4.DBD} (combination referred to as 'SS51118')

      (Zhang et al., 2022, Wang et al., 2021, Wang et al., 2020)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Silencing neurons expressing Hsap\RELAAD.VT50405 and Scer\GAL4DBD.VT007068 renders virgin females sexually unreceptive to males. Elevated levels of egg laying are seen. Activating these neurons has no effect effect on virgin receptivity, but drastically increases receptivity after mating compared with controls.

      Silencing neurons expressing Hsap\RELAAD.VT50405 and Scer\GAL4DBD.VT045154 reduces the sexual receptivity of virgin females to males. Elevated levels of egg laying are seen. Activating these neurons has no effect effect on virgin receptivity and does not increase receptivity after mating compared with controls.

      Silencing Ecol\lexAVT003280-expressing neurons does not suppress the increased receptivity to males seen in mated females when SAG-1 neurons (Hsap\RELAAD.VT50405 and Scer\GAL4DBD.VT007068-expressing) have been activated.

      Activation of SAG-1 neurons (Hsap\RELAAD.VT50405 and Scer\GAL4DBD.VT007068-expressing) in virgin females in which the Ecol\lexAVT003280-expressing neurons have been silenced only partially restores the loss of receptivity.

      (Feng et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (8)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Referred to as SAG-1 when in combination with Scer\GAL4DBD.VT007068 and SAG-2 when in combination with Scer\GAL4DBD.VT045154.

      (Feng et al., 2014)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Hsap\RELAAD.VT050405.T:Zzzz\Zip+
      Hsap\RELAAD.VT050405
      Hsap\RELAAD.VT50405
      Name Synonyms
      Secondary FlyBase IDs
        References (9)