FlyBase Allele Report: Dmel\robo2[ΔC.10xUAS.Tag:HA,Tag:SS(wg)]

General Information

Symbol

Dmel\robo2^{ΔC.10xUAS.Tag:HA,Tag:SS(wg)}

Species

D. melanogaster

Name

FlyBase ID

FBal0316478

Feature type

allele

Associated gene

Dmel\robo2

Associated Insertion(s)

Carried in Construct

M{10xUAS-HA-robo2.ΔC}

Key Links

Transgenic product class

inframe_deletion

(Evans et al., 2015)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Evans et al., 2015)

Mutagen

in vitro construct

(Evans et al., 2015)

Progenitor genotype

Carried in construct

M{10xUAS-HA-robo2.ΔC}

Cytology

Description

Ten copies of the UAS regulatory sequence drive expression of a deleted robo2 coding sequence (lacks amino acid residues 84-1022, numbering according to GenBank AAF51375) which is tagged at the N-terminal end with three copies of the Tag:HA tag. 5'UTR and signal sequences from the wg gene are also present.

(Evans et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Tagged with

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^ap.PU

(Evans et al., 2015)

abnormal neuroanatomy, with Scer\GAL4^elav.PLu

(Evans et al., 2015)

abnormal neuroanatomy, with Scer\GAL4^sli.PS

(Evans et al., 2015)

Phenotype Manifest In

intermediate longitudinal fascicle, with Scer\GAL4^elav.PLu

(Evans et al., 2015)

larval longitudinal connective, with Scer\GAL4^sli.PS

(Evans et al., 2015)

lateral longitudinal fascicle, with Scer\GAL4^elav.PLu

(Evans et al., 2015)

medial longitudinal fascicle, with Scer\GAL4^elav.PLu

(Evans et al., 2015)

symmetrical commissure, with Scer\GAL4^sli.PS

(Evans et al., 2015)

Detailed Description

Statement

Reference

Expression of robo2^{ΔC.10xScer\UAS.T:Ivir\HA1,T:SS-wg} under the control of Scer\GAL4^elav.PLu in embryos results in ectopic crossing of the midline by Fas2-positive axons in 100% of segments. All three Fas2-positive pathways collapse at the midline in nearly every segment.

Expression of robo2^{ΔC.10xScer\UAS.T:Ivir\HA1,T:SS-wg} under the control of Scer\GAL4^ap.PU in embryos results in low levels of ectopic crossing of the midline by ap-expressing neurons.

Expression of robo2^{ΔC.10xScer\UAS.T:Ivir\HA1,T:SS-wg} under the control of Scer\GAL4^sli.PS in embryos results in ectopic crossing of the midline by Fas2-positive axons in almost all segments. Fused commissures are seen in approximately 80% of segments.

(Evans et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

97242

y¹ w^*; M{10xUAS-HA-robo2.ΔC}ZH-86Fb

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

robo2^{ΔC.10xScer\UAS.T:Ivir\HA1,T:SS-wg}

robo2^{ΔC.10xUAS.Tag:HA,Tag:SS(wg)}

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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