FB2024_03 , released June 25, 2024
Allele: Avic\GFP10xUAS.smGdP.Tag:Myr(Src64B),Tag:StrepII
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General Information
Symbol
Avic\GFP10xUAS.smGdP.Tag:Myr(Src64B),Tag:StrepII
Species
A. victoria
Name
FlyBase ID
FBal0305088
Feature type
allele
Associated gene
Avic\GFP
Associated Insertion(s)
Carried in Construct
P{10xUAS-IVS-myr::smGdP-StrepTag2}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Nern et al., 2015)
Progenitor genotype
Carried in construct
P{10xUAS-IVS-myr::smGdP-StrepTag2}
Cytology
Description

Ten copies of the UAS regulatory sequence drive expression of a non-fluorescent 'spaghetti monster GFP' ('smGdP') marker which consists of 10 copies of the Tag:StrepII tag inserted in groups into the backbone of the superfolder green fluorescent protein, which has been rendered non-fluorescent by mutation. A N-terminal myristoylation signal (Tag:Myr(Src64B)) is also present, to target the protein to the plasma membrane, and the marker has been optimised for Drosophila codon usage.

(Nern et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Avic\GFP
Tagged with
Tag:Myr(Src64B)
Tag:StrepII
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Avic\GFP10xScer\UAS.smGdP.T:Myr-Src64B,T:Zzzz\StrepII
      Avic\GFP10xUAS.smGdP.Tag:Myr(Src64B),Tag:StrepII
      Name Synonyms
      Secondary FlyBase IDs
        References (1)