FB2024_03 , released June 25, 2024
Allele: Hsap\RELAAD.R82F12
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General Information
Symbol
Hsap\RELAAD.R82F12
Species
H. sapiens
Name
FlyBase ID
FBal0304334
Feature type
allele
Associated gene
Hsap\RELA
Associated Insertion(s)
Carried in Construct
P{R82F12-p65.AD}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Tuthill et al., 2013)
Progenitor genotype
Carried in construct
P{R82F12-p65.AD}
Cytology
Description

The R82F12 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

(Tuthill et al., 2013)
Allele components
Component
Use(s)
Regulatory region(s)
GMR82F12
Encoded product / tool
p65(AD)::Zip+
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      lamina monopolar neuron L2

      Comment: when combined with P{R75H08-GAL4.DBD}

      (Tuthill et al., 2013)
      lamina monopolar neuron L2 | subset

      Comment: when combined with P{R75H07-GAL4.DBD} (combination referred to as 'L2a-Gal4')

      (Yuan et al., 2021)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Silencing of L2 lamina output neurons, using Hsap\RELAAD.R82F12.T:Zzzz\Zip+, Scer\GAL4DBD.R75H08.T:Zzzz\Zip- and Hsap\KCNJ2Scer\UAS.T:Avic\GFP-EGFP, significantly affects fly responses to many different visual behavioural conditions.

      Silencing of L2 lamina output neurons, using Hsap\RELAAD.R82F12.T:Zzzz\Zip+, Scer\GAL4DBD.R75H08.T:Zzzz\Zip- and Hsap\KCNJ2Scer\UAS.T:Avic\GFP-EGFP, impairs fly responses to monocular progressive but not regressive motion.

      Silencing of L2 lamina output neurons, using Hsap\RELAAD.R82F12.T:Zzzz\Zip+, Scer\GAL4DBD.R75H08.T:Zzzz\Zip- and Hsap\KCNJ2Scer\UAS.T:Avic\GFP-EGFP, results in enhancement of reverse-optomotor inversion at high speeds.

      Silencing of L2 lamina output neurons, using Hsap\RELAAD.R82F12.T:Zzzz\Zip+, Scer\GAL4DBD.R75H08.T:Zzzz\Zip- and Hsap\KCNJ2Scer\UAS.T:Avic\GFP-EGFP, compresses the height of the contrast sensitivity tuning function.

      (Tuthill et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Split-GAL4 transgene, constructed as described in FBrf0212052.

      (Tuthill et al., 2013)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Hsap\RELAAD.R82F12.T:Zzzz\Zip+
      Hsap\RELAAD.R82F12
      Name Synonyms
      Secondary FlyBase IDs
        References (7)