The R48A08 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.
Comment: when combined with P{R66A01-GAL4.DBD}
when combined with P{R66A01-GAL4.DBD} (combination referred to as 'SS00691')
Comment: when combined with P{R29G11-GAL4.DBD}
when combined with P{R66A01-GAL4.DBD} (combination referred to as 'L1-Gal4')
when combined with P{R66A01-GAL4.DBD}
Comment: when combined with P{R29G11-GAL4.DBD}
Silencing of L1 + L2 lamina output neurons, using Hsap\RELAAD.R48A08.T:Zzzz\Zip+, Scer\GAL4DBD.R29G11.T:Zzzz\Zip- and Hsap\KCNJ2Scer\UAS.T:Avic\GFP-EGFP, significantly affects fly responses to many different visual behavioural conditions.
Silencing of L1 lamina output neurons, using Hsap\RELAAD.R48A08.T:Zzzz\Zip+, Scer\GAL4DBD.R66A01.T:Zzzz\Zip- and Hsap\KCNJ2Scer\UAS.T:Avic\GFP-EGFP, significantly affects fly responses to many different visual behavioural conditions.
Silencing of L1 lamina output neurons, using Hsap\RELAAD.R48A08.T:Zzzz\Zip+, Scer\GAL4DBD.R66A01.T:Zzzz\Zip- and Hsap\KCNJ2Scer\UAS.T:Avic\GFP-EGFP, compresses the height of the contrast sensitivity tuning function.
Split-GAL4 transgene, constructed as described in FBrf0212052.