The attP cassette in the Mi{MIC}Shaw^MI01735 insertion has been replaced with a Trojan dVP16AD cassette that contains a splice acceptor site followed by the T2A peptide, the Hsim\VP16 activation domain fused to the T:Zzzz\Zip+ component of the SplitGAL4 system ("dVP16AD") and an Hsp70 transcription termination signal. The insertion is in the correct orientation to gene trap Shaw. The splice acceptor ensures that the T2A-dVP16AD open reading frame is incorporated into the Shaw mRNA, while the T2A sequence is expected to truncate the native Shaw gene product and promotes the separate translation of the dVP16AD open reading frame. Thus expression of the Hsim\VP16 activation domain fused to the T:Zzzz\Zip+ component of the SplitGAL4 system is driven by the native Shaw regulatory sequences.