FB2024_03 , released June 25, 2024
Allele: Scer\GAL4Gad1-MI09277-TG4.2
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General Information
Symbol
Scer\GAL4Gad1-MI09277-TG4.2
Species
S. cerevisiae
Name
FlyBase ID
FBal0304252
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Mi{Trojan-GAL4.2}Gad1MI09277-TG4.2
Carried in Construct
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
phiC31 integrase
(Diao et al., 2015)
Progenitor genotype
Mi{MIC}Gad1MI09277
(Diao et al., 2015)
Associated Insertion(s)
Mi{Trojan-GAL4.2}Gad1MI09277-TG4.2
Cytology
Description

The attP cassette in the Mi{MIC}Gad1MI09277 insertion has been replaced with a Trojan GAL4 cassette that contains a splice acceptor site followed by the T2A peptide, the Scer\GAL4 coding sequence and an Hsp70 transcription termination signal. The insertion is in the correct orientation to gene trap Gad1. The splice acceptor ensures that the T2A-Scer\GAL4 open reading frame is incorporated into the Gad1 mRNA, while the T2A sequence is expected to truncate the native Gad1 gene product and promotes the separate translation of the Scer\GAL4 open reading frame. Thus Scer\GAL4 expression is driven by the native Gad1 regulatory sequences.

(Diao et al., 2015)
Allele components
Component
Use(s)
Inserted element
Mi{Trojan-GAL4.2}
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4Gad1-MI09277-TG4.2
      Name Synonyms
      Secondary FlyBase IDs
        References (3)