The attP cassette in the Mi{MIC}GluRIIEMI01909 insertion has been replaced with a Trojan GAL4 cassette that contains a splice acceptor site followed by the T2A peptide, the Scer\GAL4 coding sequence and an Hsp70 transcription termination signal. The insertion is in the correct orientation to gene trap GluRIIE. The splice acceptor ensures that the T2A-Scer\GAL4 open reading frame is incorporated into the GluRIIE mRNA, while the T2A sequence is expected to truncate the native GluRIIE gene product and promotes the separate translation of the Scer\GAL4 open reading frame. Thus Scer\GAL4 expression is driven by the native GluRIIE regulatory sequences.
ScerGAL4GluRIIE-MI01909-TG4.1 drives expression in larval bodywall muscles, and in adult ventral abdominal muscles.