The RMCE cassette of the Mi{MIC}Gycβ100B^MI01568 insertion has been replaced with a Trojan-GAL4 cassette. The insertion is in a coding intron of Gycβ100B and is predicted to gene trap the gene. The insertion is also within stops, in a coding intron, and is not predicted to gene trap this gene.

The attP cassette in the Mi{MIC}Gycβ100B^MI01568 insertion has been replaced with a Trojan GAL4 cassette that contains a splice acceptor site followed by the T2A peptide, the Scer\GAL4 coding sequence and an Hsp70 transcription termination signal. The insertion is in the correct orientation to gene trap Gycβ100B. The splice acceptor ensures that the T2A-Scer\GAL4 open reading frame is incorporated into the Gycβ100B mRNA, while the T2A sequence is expected to truncate the native Gycβ100B gene product and promotes the separate translation of the Scer\GAL4 open reading frame. Thus Scer\GAL4 expression is driven by the native Gycβ100B regulatory sequences. The Mi{Trojan-GAL4.2}Gycβ100B^{MI01568-TG4.2} insertion is also within a coding intron of the nested stops gene, but the inserted Mi{Trojan-GAL4.2} element is in the wrong orientation to gene trap the stops locus.