The attP cassette in the Mi{MIC}Rdl^MI02957 insertion has been replaced with a Trojan p65AD cassette that contains a splice acceptor site followed by the T2A peptide, the Hsap\RELA activation domain fused to the T:Zzzz\Zip+ component of the SplitGAL4 system ("p65AD") and an Hsp70 transcription termination signal. The insertion is in the correct orientation to gene trap Rdl. The splice acceptor ensures that the T2A-p65AD open reading frame is incorporated into the Rdl mRNA, while the T2A sequence is expected to truncate the native Rdl gene product and promotes the separate translation of the p65AD open reading frame. Thus expression of the Hsap\RELA activation domain fused to the T:Zzzz\Zip+ component of the SplitGAL4 system is driven by the native Rdl regulatory sequences.