The R14B07 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.
Comment: when combined with P{R64B03-p65.AD}
Comment: when combined with P{R64B03-p65.AD} (combination referred to as 'L3a-Gal4')
Silencing of L3 lamina neurons, using Hsap\RELAAD.R64B03.T:Zzzz\Zip+, Scer\GAL4DBD.R14B07.T:Zzzz\Zip- and Hsap\KCNJ2Scer\UAS.T:Avic\GFP-EGFP, extends the height of the contrast sensitivity tuning function.
Split-GAL4 transgene, constructed as described in FBrf0212052.