A 252bp Burs promoter fragment drives expression of a GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).
Activation of the B[[SEG]] neurons using the combination of the Hsim\VP1699 and Scer\GAL4DBD.Burs.T:Zzzz\Zip- split-gal4 hemidrivers results in newly emerged flies placed in a confined environment showing abdominal flexion behaviours characteristic of wing expansion. The flies also show normal tanning.
Inhibition of the B[[SEG]] neurons using the combination of the Hsim\VP1699 and Scer\GAL4DBD.Burs.T:Zzzz\Zip- split-gal4 hemidrivers results in newly emerged flies placed in a confined environment failing to expand their wings and failing to show the abdominal flexion behaviours characteristic of wing expansion.
Activation of the B[[AG]] neurons using the combination of the Hsim\VP16N9A88A and Scer\GAL4DBD.Burs.T:Zzzz\Zip- split-gal4 hemidrivers does not result in the rapid induction of abdominal flexion behaviours characteristic of wing expansion in newly emerged flies placed in a confined environment. The wings become malleable and partially unfolded in response to occasional grooming by the hindlegs. The flies also show normal tanning.
Inhibition of the B[[AG]] neurons using the combination of the Hsim\VP16N9A88A and Scer\GAL4DBD.Burs.T:Zzzz\Zip- split-gal4 hemidrivers does not prevent newly emerged flies placed in a confined environment showing the abdominal flexion behaviours characteristic of wing expansion.