FlyBase Allele Report: Dmel\rig[UAS.ECFP]

General Information

Symbol

Dmel\rig^UAS.ECFP

Species

D. melanogaster

Name

FlyBase ID

FBal0294750

Feature type

allele

Associated gene

Dmel\rig

Associated Insertion(s)

Carried in Construct

P{UAS-rig.eCFP}

Key Links

Transgenic product class

wild_type

(Borg and Cauchi, 2013)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Borg and Cauchi, 2013)

Mutagen

in vitro construct

(Borg and Cauchi, 2013)

Progenitor genotype

Carried in construct

P{UAS-rig.eCFP}

Cytology

Description

UASt regulatory sequences drive expression of rig tagged at the C-terminal end with ECFP.

(Borg and Cauchi, 2013)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

rig

Tagged with

ECFP

cyan fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval muscle system | larval stage, with Scer\GAL4^da.PU

(Borg et al., 2015)

Detailed Description

Statement

Reference

Expression of rig^{Scer\UAS.T:Avic\GFP-ECFP} under the control of Scer\GAL4^Mef2.PR has no effect on flight ability of adult flies, mobility of the third instar larvae or shape of pupae.

(Lanfranco et al., 2017)

Expression of rig^{Scer\UAS.T:Avic\GFP-ECFP} driven by Scer\GAL4^da.PU induces supernumerary bodies that are largely nuclear in larval muscles; these foci are reduced and mostly restricted to cytoplasm in a rig⁰⁵⁰⁵⁶/rig^k07917 background. Expression of rig^{Scer\UAS.T:Avic\GFP-ECFP} driven by Scer\GAL4^Mef2.PR does not significantly affect flight or lifespan.

(Borg et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Gem3^ΔN.UAS, Scer\GAL4^Mef2.PR, rig^UAS.ECFP has abnormal locomotor behavior | third instar larval stage phenotype

(Lanfranco et al., 2017)

Gem3^ΔN.UAS, Scer\GAL4^Mef2.PR, rig^UAS.ECFP has abnormal size | pupal stage phenotype

(Lanfranco et al., 2017)

Gem3^ΔN.UAS, Scer\GAL4^Mef2.PR, rig^UAS.ECFP has flightless phenotype

(Borg et al., 2015)

Gem3^ΔN.UAS, Scer\GAL4^Mef2.PR, rig^UAS.ECFP has short lived phenotype

(Borg et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Simultaneous expression of Gem3^ΔN.Scer\UAS and rig^{Scer\UAS.T:Avic\GFP-ECFP} leads to strongly reduced mobility in third instar larvae and more elongated shape of the puparium with the Scer\GAL4^Mef2.PR driver and to complete adult lethality with Scer\GAL4^elav-C155.

(Lanfranco et al., 2017)

Co-expression of Gem3^ΔN.Scer\UAS and rig^{Scer\UAS.T:Avic\GFP-ECFP} driven by Scer\GAL4^Mef2.PR results in flightless flies with shortened lifespans (compared to either single expression, which is similar to controls).

(Borg et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

rig^UAS.ECFP/Scer\GAL4^1032.hx rescues rig^NIG.11171R

(Borg and Cauchi, 2013)

Partially rescues

rig^UAS.ECFP/Scer\GAL4^da.PU partially rescues rig⁰⁵⁰⁵⁶

(Borg et al., 2015)

rig^UAS.ECFP/Scer\GAL4^da.PU partially rescues rig⁰⁵⁰⁵⁶/rig^k07917