FlyBase Allele Report: Dmel\Marf[HMC03883]

General Information

Symbol

Dmel\Marf^HMC03883

Species

D. melanogaster

Name

FlyBase ID

FBal0294441

Feature type

allele

Associated gene

Dmel\Marf

Associated Insertion(s)

Carried in Construct

P{TRiP.HMC03883}

Also Known As

UAS-Marf RNAi, Marf RNAi, TRiP.HMC03883

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMC03883}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMC03883

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Friedreich ataxia 1

modeled by fh^RNAi.UAS.cAa

(Edenharter et al., 2018)

exacerbates frontotemporal dementia and/or amyotrophic lateral sclerosis 2

modeled by Chchd2^S81L.UAS

(Baek et al., 2021)

ameliorates Parkinson's disease 6

modeled by Pink1^B9

(Li et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

A band | adult stage | progressive, with Scer\GAL4^Mhc.PW

(Li et al., 2016)

axon | adult stage, with Scer\GAL4^VGlut-OK371

(Smith et al., 2019)

dorsal longitudinal indirect flight muscle cell | adult stage | progressive, with Scer\GAL4^Mhc.PW

(Li et al., 2016)

embryonic/larval hypodermal muscle cell | third instar larval stage, with Scer\GAL4^Mef2.PU

(Deng et al., 2016)

female germline stem cell, with Scer\GAL4^nanos.PG

(Taslim et al., 2023)

indirect flight muscle | adult stage, with Scer\GAL4^Mef2.PR

(Katti et al., 2022)

indirect flight muscle cell, with Scer\GAL4^Mhc.PU

(Li et al., 2023)

indirect flight muscle cell | adult stage, with Scer\GAL4^Mhc.PU

(Deng et al., 2016)

mitochondrion | adult stage, with Scer\GAL4^Mef2.PR

(Katti et al., 2022)

mitochondrion | adult stage, with Scer\GAL4^Mhc.PU

(Li et al., 2023, Deng et al., 2016)

mitochondrion | adult stage, with Scer\GAL4^VGlut-OK371

(Smith et al., 2019)

mitochondrion | adult stage | progressive, with Scer\GAL4^Mhc.PW

(Li et al., 2016)

mitochondrion | third instar larval stage, with Scer\GAL4^Mef2.PU

(Deng et al., 2016)

myotube | adult stage | progressive, with Scer\GAL4^Mhc.PW

(Li et al., 2016)

ommatidium, with Scer\GAL4^GMR.long

(Zhao and Wang, 2020)

sarcomere | adult stage | progressive, with Scer\GAL4^Mhc.PW

(Li et al., 2016)

Z disc | adult stage | progressive, with Scer\GAL4^Mhc.PW

(Li et al., 2016)

Detailed Description

Statement

Reference

Expression of Marf^HMC03883 under the control of Scer\GAL4^GMR.long leads to fewer endoplasmic reticulum-mitochondria contact sites in ommatidia.

(Zhao and Wang, 2020)

Expression of Marf^HMC03883 under the control of Scer\GAL4^VGlut-OK371 significantly reduces mitochondrial length in axons of adult neurons, compared to controls, with no effect on mitochondrial number, compared to controls

(Smith et al., 2019)

Expression of Marf^HMC03883 leads to strong defects in mitochondrial fusion in the body wall cells of third instar larvae (with the Scer\GAL4^Mef2.PU driver) as well as in the indirect flight muscles in adults (with the Scer\GAL4^Mhc.PU driver) - the mitochondria are short and fragmented compared to controls.

(Deng et al., 2016)

The expression of Marf^HMC03883 under the control of Scer\GAL4^Mhc.PW results in a range of defects in dorsal longitudinal indirect flight muscles, as compared to controls: thinner myofibrils and sarcomere disorganization (with vanishing Z lines and A bands) at 21, but not at 4, days post eclosion; mitochondrion defects, as shown by an abnormal morphology and a significant and progressive decrease in the mitochondria electron density in electron microscopy images at days 14 to 21 days post eclosion; and a significant increase in cytoplasmic polyubiquitin aggregates at 14 days post eclosion. These individuals also exhibit flight defects, as shown by a significant decrease in landing height at 21 days post eclosion, as compared to controls.

(Li et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Marf^HMC03883, Scer\GAL4^VGlut-OK371 has abnormal neuroanatomy | adult stage phenotype, non-enhanceable | somatic clone by gfzf⁵⁴¹

(Smith et al., 2019)

NOT suppressed by